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Objective:To evaluate the accuracy of mini-fluid challenge test in predicting fluid responsiveness in elderly patients undergoing surgery in prone position.Methods:Forty-eight elderly patients, aged ≥ 65 yr, with body mass index of 18-30 kg/m 2, of American Society of Anesthesiologists physical status Ⅱ or Ⅲ, scheduled for elective spinal surgery, were enrolled.Patients were mechanically ventilated using a volume-controlled mode with a tidal volume of 6 ml/kg of ideal body weight during operation.A radial arterial catheter was inserted and connected to FloTrac/Vigileo system to monitor hemodynamic parameters.At 5 min after prone position, volume expansion was started when the hemodynamics was stable: lactated Ringer′s solution 1 ml/kg was intravenously infused over 1 min, 1 min later lactated Ringer′s solution 1 ml/kg was intravenously infused over 1 min, 1 min later lactated Ringer′s solution 1 ml/kg was intravenously infused over 1 min, and 1 min later operation was started.After tracheal intubation and before prone position (T 1), 5 min of prone position (T 2), 1 min after 1st infusion of 1 ml/kg liquid (T 3), and 1 min after 2nd infusion of 1 ml/kg liquid (T 4) and 1 min after infusion of 3 ml/kg fluid (T 5), heart rate, mean arterial pressure, cardiac output, cardiac index, stroke volume, stroke volume index (SVI), stroke volume variability (SVV), pulse pressure variability (PPV), and changes in SVI induced by rapid infusion of 1, 2 and 5 ml/kg crystalloid (ΔSVI 1 ml/kg, ΔSVI 2 ml/kg, ΔSVI 5 ml/kg) were calculated.Positive fluid challenges were defined as an increase in SVI of 10% or more from baseline, and the patients were divided into responder group (R) and non-responder group (NR). Receiver operating characteristic curves predicting fluid responsiveness were generated for ΔSVI 1 ml/kg, ΔSVI 2 ml/kg, SVV and PPV, and areas under the receiver operating characteristic curves (AUC) were calculated. Results:Thirty patients were enrolled in group R and 18 cases in group NR.The AUC of ΔSVI 1 ml/kg in predicting fluid volume responsiveness was 0.87 with a diagnostic threshold of 7%, a sensitivity of 80%, and a specificity of 83%.The AUC of ΔSVI 2 ml/kg in predicting fluid responsiveness was 0.928 with a diagnostic threshold of 8%, a sensitivity of 78%, and a specificity of 89%.The AUC of SVV and PPV in predicting fluid responsiveness was 0.65 and 0.53, respectively. Conclusion:Mini-fluid challenge test guided by ΔSVI can predict fluid responsiveness in elderly patients undergoing surgery in prone position, and rapid infusion of 2 ml/kg crystalloid provides better accuracy than 1 ml/kg.
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Objective@#To investigate the clinical relevant factors associated with transfusion associated necrotizing enterocolitis (TANEC) in order to reduce the incidence of neonatal necrotizing enterocolitis (NEC).@*Methods@#The clinical data of neonates admitted to the First Hospital of Lanzhou University and received blood transfusion therapy from January 2017 to June 2018 were collected, including perinatal factors, basic conditions of children, and comorbidities.According to the occurrence or absence of NEC within 48 hours after transfusion, the patients were divided into TANEC group and no-TANEC group.The clinical data of the two groups were compared.@*Results@#Univariate analysis showed that there were statistical differences (P<0.05) in the mode of delivery, gestational age, birth weight, neonatal sepsis, patent ductus arteriosus(PDA), neonatal respiratory distress syndrome (NRDS), and anemia.The logistic multivariate analysis revealed that gestational age(P<0.05, OR=0.772, 95%CI: 0.684-0.871), the birth weight(P<0.05, OR=0.236, 95%CI: 0.079-0.711)were protect fators of TANEC, the degree of anemia(mode 1: P<0.05, OR=3.129, 95%CI: 1.003-9.756; mode 2: P<0.05, OR=3.449, 95%CI: 1.024-11.609)and sepsis (mode 1: P<0.05, OR=6.327, 95%CI: 1.732-23.720; mode 2: P<0.05, OR=8.154, 95%CI: 2.122-31.336)were significant risk fators for TANEC.@*Conclusion@#The factors leading to the occurrence of TANEC are various.When transfused, the greater the gestational age, the higher the birth weight, the lower the risk of developing NEC.The more severe the combination of neonatal sepsis and anemia, the higher the risk of developing TANEC.Clinically, comprehensive measures should be taken to prevent neonatal anemia.According to the specific conditions of the children and the degree of anemia, a reasonable clinical strategy should be formulated to avoid blood transfusion to reduce the incidence of TANEC and improve the prognosis of the children.
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Objective:To evaluate the characteristics and risk factors for blood transfusion in very low birth weight infants(VLBWI).Methods:Clinical data of one hundred VLBWI, hospitalized from July, 2016 to June, 2019, were studied retrospectively.The infants were divided into two groups according to whether they received blood transfusion.The general information, incidence of diseases and treatment measures were compared between two groups.The risk factors influencing the blood transfusion were analyzed.Results:Of the one hundred VLBWI, sixty-nine cases needed blood transfusion.The first time of blood transfusion ranged from one to four weeks after birth, and average number of transfusions was 6 times.Maternal anemia during pregnancy, birth weight, gestational age, hemoglobin and hematocrit at birth, volume of blood taking within two weeks after birth, duration of hospitalization, duration of paraenteral nutrition, delivery method, need for intubation and neonatal respiratory distress syndrome, apnea, bronchopulmonary dysplasia, patent ductus arteriosus showed significant differences between the two groups( P<0.05). Logistic regression analysis revealed that lower gestation( OR=0.386, 95% CI 0.212-0.704, P=0.002), longer duration of hospital stay( OR=2.177, 95% CI 1.170-4.049, P=0.014), prolonged parenteral nutrition( OR=1.195, 95% CI 1.083-1.319, P<0.001), greater volume of blood taking within two weeks after birth ( OR=1.269, 95% CI 1.083-1.487, P=0.003)and cesarean delivery( OR=5.513, 95% CI 1.056-28.770, P=0.043) were associated with increasing risk of blood transfusion in VLBWI. Conclusion:The gestational age, length of hospital stay, blood intake within two weeks after birth, duration of paraenteral nutrition and delivery method all affected the risk of blood transfusion to varying degrees.
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Objective:To investigate the clinical relevant factors associated with transfusion associated necrotizing enterocolitis (TANEC) in order to reduce the incidence of neonatal necrotizing enterocolitis (NEC).Methods:The clinical data of neonates admitted to the First Hospital of Lanzhou University and received blood transfusion therapy from January 2017 to June 2018 were collected, including perinatal factors, basic conditions of children, and comorbidities.According to the occurrence or absence of NEC within 48 hours after transfusion, the patients were divided into TANEC group and no-TANEC group.The clinical data of the two groups were compared.Results:Univariate analysis showed that there were statistical differences ( P<0.05) in the mode of delivery, gestational age, birth weight, neonatal sepsis, patent ductus arteriosus(PDA), neonatal respiratory distress syndrome (NRDS), and anemia.The logistic multivariate analysis revealed that gestational age( P<0.05, OR=0.772, 95% CI: 0.684-0.871), the birth weight( P<0.05, OR=0.236, 95% CI: 0.079-0.711)were protect fators of TANEC, the degree of anemia(mode 1: P<0.05, OR=3.129, 95% CI: 1.003-9.756; mode 2: P<0.05, OR=3.449, 95% CI: 1.024-11.609)and sepsis (mode 1: P<0.05, OR=6.327, 95% CI: 1.732-23.720; mode 2: P<0.05, OR=8.154, 95% CI: 2.122-31.336)were significant risk fators for TANEC. Conclusion:The factors leading to the occurrence of TANEC are various.When transfused, the greater the gestational age, the higher the birth weight, the lower the risk of developing NEC.The more severe the combination of neonatal sepsis and anemia, the higher the risk of developing TANEC.Clinically, comprehensive measures should be taken to prevent neonatal anemia.According to the specific conditions of the children and the degree of anemia, a reasonable clinical strategy should be formulated to avoid blood transfusion to reduce the incidence of TANEC and improve the prognosis of the children.
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Objective:To systematically evaluate the clinical efficacy of oral ginger capsule or ginger powder in chemotherapy-induced nausea and vomiting in cancer patients.Methods:Computers searched Chinese Journal Full-text Database (CNKI), China Biomedical Literature Database (CBM), Wanfang Database, PubMed, EMbase, Web of Science, and Cochrane Library about oral chemotherapy in patients with cancer ginger correlation clinical curative effect of nausea and vomiting randomized controlled trial, supplemented by other search methods, the time range was built until July 2019. Quality evaluation and data extraction were performed independently by two investigators, and Meta analysis was performed by RevMan5.3 software.Results:A total of 12 articles and 13 studies were included, with a total of 1 105 patients. Meta-analysis showed that oral ginger capsule or ginger powder reduced the incidence of acute vomiting (risk ratio value was 0.76, 95% confidence interval was 0.59-0.98, P<0.05) and the severity of vomiting (mean difference value was-0.79, 95% confidence interval was-1.36--0.23, P<0.01), including the severity of acute vomiting (mean difference value was-1.39, 95% confidence interval was-2.72--0.06, P<0.05) and the severity of delayed vomiting (mean difference value was-0.46, 95% confidence interval was-0.82--0.10, P<0.05). However, there was no significant difference between the two groups in the incidence and severity of acute and delayed nausea ( P>0.05). Conclusions:This study demonstrates that oral ginger capsule or ginger powder is a complementary treatment for chemotherapy-induced nausea and vomiting in cancer patients, and more high-quality studies are needed to validate its clinical efficacy in the future.
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Objective:To systematically evaluate the clinical efficacy of oral ginger capsule or ginger powder in chemotherapy-induced nausea and vomiting in cancer patients.Methods:Computers searched Chinese Journal Full-text Database (CNKI), China Biomedical Literature Database (CBM), Wanfang Database, PubMed, EMbase, Web of Science, and Cochrane Library about oral chemotherapy in patients with cancer ginger correlation clinical curative effect of nausea and vomiting randomized controlled trial, supplemented by other search methods, the time range was built until July 2019. Quality evaluation and data extraction were performed independently by two investigators, and Meta analysis was performed by RevMan5.3 software.Results:A total of 12 articles and 13 studies were included, with a total of 1 105 patients. Meta-analysis showed that oral ginger capsule or ginger powder reduced the incidence of acute vomiting (risk ratio value was 0.76, 95% confidence interval was 0.59-0.98, P<0.05) and the severity of vomiting (mean difference value was-0.79, 95% confidence interval was-1.36--0.23, P<0.01), including the severity of acute vomiting (mean difference value was-1.39, 95% confidence interval was-2.72--0.06, P<0.05) and the severity of delayed vomiting (mean difference value was-0.46, 95% confidence interval was-0.82--0.10, P<0.05). However, there was no significant difference between the two groups in the incidence and severity of acute and delayed nausea ( P>0.05). Conclusions:This study demonstrates that oral ginger capsule or ginger powder is a complementary treatment for chemotherapy-induced nausea and vomiting in cancer patients, and more high-quality studies are needed to validate its clinical efficacy in the future.
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High mobility group box 1 protein(HMGB1) is widely present in eukaryotic nuclei and named for its rapid migration during polyacrylamide gel electrophoresis. Studies have shown that HMGB1 playes an important role in the development of gene transcription,inflammation,cancer and other diseases. In this paper, the biological characteristics of HMGB1 and its research progress in pediatric diseases are reviewed.
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Objective To investigate the expression of UCH37 in cervical cancer tissue,and the associa-tion of UCH37 expression and clinicopathological features of cervical cancer patients,and detect the effect of UCH37 on cell proliferation,cell apoptosis and cell cycle of HeLa cells. Methods Quantitative real-time poly-merase chain reaction(qRT-PCR)was conduct to detect the expression of UCH37 mRNA in cancer tissue and the adjacent tissue of cervical cancer patients. Spearman′s rank test was conduct to analyze the clinical relevance of UCH37 in cervical cancer. Cell count kit-8 was performed to detect the effect of UCH37 on proliferation of HeLa cells. Flow cytometry was performed to detect the effect of UCH37 on cell apoptosis and cell cycle of HeLa cells. Results qRT-PCR results revealed that UCH37 was upregulated in cervical cancer tissue compare to the adjacent tissue.Spearman rank correlation analysis showed that high UCH37 expression was significantly associated with FI-GO stage of cervical cancer patients.CCK-8 assay results showed that cell proliferation was inhibited in HeLa cells transfected with UCH37-siRNAs,and flow cytometry assays revealed that cell apoptosis was promoted in HeLa cells transfected with UCH37-siRNAs.Furthermore,compared with HeLa cells transfected with UCH37-NC,HeLa cells transfected with UCH37 siRNAs were arrested at G1 stage.Conclusion UCH37 expression is upregulated in tissue of cervical cancer,and UCH37 could promote cell proliferation of HeLa cells.UCH37 is a potential therapeu-tic target in cervical cancer.
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Objective To investigate the effect of serum CYFRA21-1 and SCCAg levels on the prognosis of patients with cervical cancer radiotherapy.Methods 100 cases of cervical cancer were diagnosed by pathological examination in our hospital from October 2008 and October 2011,Using radioimmunoassay(RIA) in detection of serum CYFRA21-1 and SCCAg level,Pearson and Spearman correlation is used to study the relationship between serum CYFRA21-1 and SCCAg level and cervical cancer patients with clinical features,using the Cox regression model of CYFRA21-1 and SCCAg level and uterine cervix cancer death or recurrence and metastasis of the relationship.Results 100 cases of patients with cervical cancer,endpoint events in 21 cases,LNR4 patients appear higher risk of endpoint events about 44.3%,CYFRA21-1 and SCCAg levels were with cervical cancer patients with CA-125 values,tumor staging,depth of tumor invasion into positive correlation,and the age of patients,no significant correlation,tumor differentiation(P=0.013),serum CYFRA21-1 levels(P=0.02),serum SCCAg levels(P=0.03),depth of tumor invasion(P=0.04) is cervical cancer patients with endpoint events independent risk factors.Conclusion CYFRA21-1 and SCCAg levels were closely related to the prognosis of patients with cervical cancer radiotherapy.
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A novel electrochemiluminescence ( ECL) method for the determination of L-cysteine ( L-Cys) was established. water-soluble CdS quantum dots ( QDs) with Cd2+rich surface were synthesized via a controllable one-poe approach. The mercapto group in L-cysteine molecule can specifically interact with excessive Cd2+on the surface of CdS QDs, resulting in enhancement of ECL intensity of the CdS QDs, which can be used for the detection of L-Cys. Under the optimal experimental conditions, the enhancement of ECL intensity was linear with the concentration of L-Cys in the range of 5. 0×10-9-1. 0×10-5 mol/L. The limit detection of (S/N=3) was 1. 2×10-9 mol/L. In comparison with other methods for detecting L-Cys, this method is more simple and selective, and can be applied to detect L-Cys in real sample with satisfactory results.
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In the meantime of collecting data the Wechat public platform utilization of main Class Ⅲ hospitals in Wuhan area,the paper conducts contrastive analysis on the use of Wechat with Wechat top 10 public hospitals from the perspectives of basic operation situation,menu function setting,Wechat information push and public platform influence,analyzes the advantages and disadvantages of the Wechat public platform of main Class Ⅲ hospitals in Wuhan area,and provides reference for Wuhan and related areas to improve the construction,utilization and promotion of the Wechat public platform of hospitals.
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Objective:To improve students' knowledge and skills of AIDS prevention,analyze the effect of interactive peer education,and explore effective ways to carry out peer education.Methods:From September 2015 to May 2016,the freshmen in one college of Xi'an were investigated of AIDS knowledge,attitude and behavior using questionnaire as baseline data.Seven hundred peers of them received education with the methods of brainstorming,group discussion and questions,and case analysis.Then a questionnaire survey was conducted again to evaluate the effect of peer education.Results:After the intervention peer education,the scores of basic AIDS knowledge,prevention knowledge,relevant attitude and the rate of positive behavioral selection were significantly increased (P ＜0.05).Approximately 92.50％ of the students thought that having sex with only one partner can reduce the spread of AIDS and 81.76％ of the students were willing to help AIDS patients.Conclusions:Interactive peer education for college students has a good applicability,the effect is remarkable,and it is worth popularizing in colleges.
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Objective To study ultrasonic parameters characteristics of lactation mastitis,breast cancer by using color doppler flow imaging(CDFI)and ultrasonic elastography(UE),and to explore its clinical value.Methods Retrospective analysis was conducted in the clinical data of 85 female patients (a total of 85 breast lesions).All patients were confirmed by pathology,and according to the results of pathology,they were divided into non lactation mastitis group(group NLM,a total of 28 cases),breast cancer group(group Ca,a total of 57 cases).All patients accepted CDFI and UE before treatment,and compared ultrasound parameters between the two groups.Results The proportions of class 0~I(71.43%)and RI<0.7(82.14%)in group NLM were significantly higher than those in group Ca,while the proportions of classⅡ ~Ⅲ(28.57%)and RI≥0.7 (17.86%)were significantly lower than 75.44%,84.21% in group Ca(χ2 =17.185,35.217,all P<0.05).The UE ratings(1.75 ±0.97)and the strain rate ratio (1.64 ±0.83)in NLMgroup were lower than (4.19 ±0.74),(5.03 ±1.08)in group Ca(t=-12.873,-14.623,all P<0.05).The accuracy of CDFI +UE(89.41%)was higher than the accuracy of UE(76.47%)or the accuracy of CDFI(67.06%)(χ2 =12.337,P<0.05).Conclusion CDFI,UE have a certain diagnosis ability for non lactation mastitis and breast cancer,combining both can obtain better diagnostic value,which is worthy of clinical application.
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Objective:To analyze the causes and influencing factors of the strained relationship between doctors and patients and explore the corresponding countermeasures.Methods:Five public hospitals in Ningxia were selected to conduct a questionnaire survey.The influencing factors of doctor-patient relationship were analyzed using SPSS17.0,combined with the results of field case interviews.Results:The society was not satisfied with the medical staff,the rate of dissatisfaction was 87.9％.The causes of tense doctor-patient relationship were mainly focused on the following aspects:imperfect medical insurance system,unreasonable medical system,negative media reports,inflated expectations of patients,lack of necessary communication between doctors and patients,and lack of medical ethics.Conclusion:According to the influencing factors of doctor-patient relationship,this paper puts forward some countermeasures from the perspective of governments,the society and media.
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Objective To analyze National Center for Clinical Laboratories external quality assessment(EQA)of blood cell morphology,and to summarize the cell system,morphological types and frequency of EQA.Methods EXCEL software was used to analyze retrospectively 506 pictures from National Center for Clinical Laboratories 51 times EQA of blood cell morphology from 1999 to 2016,then the type and frequency were summarized.Results Among the 506 pictures in National Center for Clinical Laboratories EQA from 1999 to 2016,380 pictures came from peripheral blood,125 pictures came from bone marrow,and the ratio was 3:1.The remaining one came from cerebrospinal fluid cells.In results of 51 times EQA,there were 135 kinds of cell morphology,including 37.78% morphology once only.In blood cell types,mononuclear had the highest frequency(28 times),then atypical lymphocyte appeared 25 times.Conclusion It is necessary for each laboratory to learn the content of blood cell morphology,and master proficiently cell morphology characteristics,especially the basic cells in blood smear for the improvement of diagnosis accuracy.
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Objective To analyze National Center for Clinical Laboratories external quality assessment(EQA)of blood cell morphology,and to summarize the cell system,morphological types and frequency of EQA.Methods EXCEL software was used to analyze retrospectively 506 pictures from National Center for Clinical Laboratories 51 times EQA of blood cell morphology from 1999 to 2016,then the type and frequency were summarized.Results Among the 506 pictures in National Center for Clinical Laboratories EQA from 1999 to 2016,380 pictures came from peripheral blood,125 pictures came from bone marrow,and the ratio was 3:1.The remaining one came from cerebrospinal fluid cells.In results of 51 times EQA,there were 135 kinds of cell morphology,including 37.78% morphology once only.In blood cell types,mononuclear had the highest frequency(28 times),then atypical lymphocyte appeared 25 times.Conclusion It is necessary for each laboratory to learn the content of blood cell morphology,and master proficiently cell morphology characteristics,especially the basic cells in blood smear for the improvement of diagnosis accuracy.
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Objective To investigate the clinical efficacy of acupuncture combined with back Shu acupoint catgut embedding therapy in treating allergic rhinitis. Methods Totally 66 allergic rhinitis patients were included. Random number table method was used to divide the 66 patients into observation group and control group, with 33 cases in each group. The control group was given Ketotifen Fumarate Nasal Drops nose drops 2 drops each time, 3 times a day, Loratadine Tablets 10 mg orally, once a day for 4 weeks. The experimental group was treated with acupuncture, once a day, 10 d as a treatment course, three courses in total; at the same time, back Shu acupoint catgut embedding therapy was given, 15 d each time, twice treatment in total. After the end of treatment, clinical efficacy, clinical symptom score, life quality score, serum levels of inflammatory factors, serum immunoglobulin, peripheral blood eosinophil count and the incidence of adverse reactionsof the two groups were observed. Results The total effective rate was 93.94％ (31/33) in observation group and 75.76％ (25/33) in the control group, with statistical significance in the two groups (P0.05). Conclusion Acupuncture combined with catgut embedding therapy in the treatment of allergic rhinitis has a significant clinical efficacy, which can improve the level of inflammatory factors in patients with high safety.
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Objective To investigate the cognition and treatment rate of hormone replacement therapy (HRT)in perimenopause women.Methods A survey was conducted in 262 perimenopause women first coming to outpatients care.A predesigned structured questionnaire was used to collect information:the symptoms of perimenopa-use women,the cognition and acceptance to the treatment.Results Finally,248 women with the mean age of (50.30 ±2.10)years finished the questionnaire,the natural menopause age was (48.90 ±1.90)years.48.79% of these women understood the role of HRT in clinical application,and only 12.50% knew the side effect of HRT. Among them,35.48% thought that HRT was necessary for the duration of perimenopause.40.73% of them had the attitude to treatment with HRT.After the education of perimenopausal acknowledgement,117 patients (47.18%) accepted the treatment with HRT.Conclusion It showed that the awareness and treatment rate of HRT in perimeno-pause women was low.The health education should be strengthened to improve the level of perimenopause health care.
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Objective To determine the association of one single nucleotide polymorphism loci G 398C in PRM2 with male in‐fertility in Chinese Han population .Methods A total of 386 infertile men were recruited as the observation group and 255 fertile men were recruited as the control group .Routine semen analysis as well as sperm functional parameters such as DNA integrity and nucleoprotein maturity rates were analyzed .Direct sequencing of G398C in PRM2 gene of infertile and fertile men was also conduc‐ted to evaluate the association of G398C SNP loci with male infertility .Results Statistical analysis showed that the frequencies of CC genotype of PRM2 G398C was significantly different between the infertile (11 .92% ) and fertile men (6 .67% ) and it was asso‐ciated with increased risk of male infertility (OR= 2 .002 ,95% CI= 1 .097 -3 .653 ,P< 0 .05) .Moreover ,it was discovered that sperm DNA integrity as well as nucleoprotein maturity rate of CC genotype were dramatically decreased compared with other geno ‐types (P<0 .05) ,which would probably lead to infertility .Conclusion Our results gave the first evidence that PRM 2 G398C poly‐morphism was associated with male infertility in Chinese Han population .
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Objective:To investigate the effect of Matrine on inhibiting lipopolysaccharide( lipopolysaccharide ,LPS) -induced macrophages to secrete inflammatory cytokines,including tumor necrosis factor-α( TNF-α) ,leukocyte mediated element 1β( IL-1β) on Toll like receptor 4 ( TLR4 ) , c-jun expression. Methods: Cultured macrophage RAW264. 7 of mouse and divided into four groups, including blank control group,matrine group,LPS group,matrine intervention group. Incubated by the concentration was 100 μg/L LPS DMEM for 1 h,then the LPS was discard. Added free serum DMEM or 125. 25 mg/L matrine DMEM to culture. The cells and culture solution of 5,30,60,120 min after the treatment of the above four groups were collected respectively. Detected of mouse RAW264. 7 macrophage TLR4 and c-jun mRNA expression by PCR. Detected of mouse macrophage RAW264. 7 cells c-jun protein expression by immunocytochemical. Measured secretion in cultured solution of TNF-α and IL-1β by ELISA. Results:Expression TLR4 mRNA,c-jun mRNA and c-jun protein and TNF -α and IL-1β secretion quantity indexes of matrine induced and blank group had no statistical significant differences ( P>0. 05 ) . TLR4 mRNA, c-jun mRNA and c-jun protein expression secretion of and TNF-α and IL-1β LPS group at each time point were significantly higher than that of blank group,and the high level to maintain more than 2 h (P<0. 05);matrine intervention group could effectively inhibit LPS induced macrophage TLR4 mRNA,c-jun mRNA and c-jun protein expression and reduce the secretion of inflammatory cytokines TNF-α and IL-1β. Conclusion: Matrine may inhibit LPS induced macrophage mitogen activated protein kinase signal pathway of TLR4 and c-jun expression so that it can effectively reduce the end inflammatory cytokines TNF-α and IL-1β release,effectively inhibit the inflammatory reaction and reduce the degree of endotoxin inflammatory re-sponse.